Journal: Frontiers in Microbiology

Article Title: In silico Prediction, in vitro Antibacterial Spectrum, and Physicochemical Properties of a Putative Bacteriocin Produced by Lactobacillus rhamnosus Strain L156.4

doi: 10.3389/fmicb.2017.00876

Figure Lengend Snippet: Antibacterial activity spectrum of Lactobacillus rhamnosus LL156.4 in the deferred agar-spot and spot-on-the lawn assays .

Article Snippet: Physicochemical characterization assays were performed with the CFS of L. rhamnosus L156.4 using L. fermentum ATCC 9338 as the indicator species (Table ).

Techniques: Activity Assay

Journal: Nutrients

Article Title: In Vitro Antimicrobial Activity and Probiotic Potential of Bifidobacterium and Lactobacillus against Species of Clostridium

doi: 10.3390/nu11020448

Figure Lengend Snippet: Inhibitory activity of Bifidobacterium and Lactobacillus against Clostridium spp. based on the agar spot test.

Article Snippet: In fact, adherence values for L. plantarum ATCC 8014 were higher than those observed for L. fermentum ATCC 23271 ( , p = 0.0037).

Techniques: Activity Assay, Probiotics, Inhibition

Journal:

Article Title: An In Vitro Study of the Probiotic Potential of a Bile-Salt-Hydrolyzing Lactobacillus fermentum Strain, and Determination of Its Cholesterol-Lowering Properties

doi: 10.1128/AEM.69.8.4743-4752.2003

Figure Lengend Snippet: Bile-salt-hydrolyzing activity as detected by the plate assay method

Article Snippet: These results are shown in Table . table ft1 table-wrap mode="anchored" t5 TABLE 3. caption a7 Bile acid Amount (μmol ± SD) of CA formed by: L. fermentum KC5b S. bovis ATCC 43143 E. faecalis UK873 TCA 3.0 ± 1.2 30.9 ± 1.0 9.5 ± 6.6 GCA 3.1 ± 2.0 35.5 ± 1.3 23.9 ± 5.2 Open in a separate window a Values calculated on the basis of growth in MRS broth supplemented with 1 mM GCA and 1 mM TCA.

Techniques: Activity Assay

Journal:

Article Title: An In Vitro Study of the Probiotic Potential of a Bile-Salt-Hydrolyzing Lactobacillus fermentum Strain, and Determination of Its Cholesterol-Lowering Properties

doi: 10.1128/AEM.69.8.4743-4752.2003

Figure Lengend Snippet: Growth and deconjugation of bile acids. Changes in pH (▵) and growth (OD650 nm) (▪) of L. fermentum KC5b (A), S. bovis ATCC 43143 (B), and E. faecalis UK873 (C) in MRS broth supplemented with 1 mM TCA (□) and 1 mM GCA (▴) are shown. Each point represents the mean of three trials; error bars represent standard deviations.

Article Snippet: These results are shown in Table . table ft1 table-wrap mode="anchored" t5 TABLE 3. caption a7 Bile acid Amount (μmol ± SD) of CA formed by: L. fermentum KC5b S. bovis ATCC 43143 E. faecalis UK873 TCA 3.0 ± 1.2 30.9 ± 1.0 9.5 ± 6.6 GCA 3.1 ± 2.0 35.5 ± 1.3 23.9 ± 5.2 Open in a separate window a Values calculated on the basis of growth in MRS broth supplemented with 1 mM GCA and 1 mM TCA.

Techniques:

Journal:

Article Title: An In Vitro Study of the Probiotic Potential of a Bile-Salt-Hydrolyzing Lactobacillus fermentum Strain, and Determination of Its Cholesterol-Lowering Properties

doi: 10.1128/AEM.69.8.4743-4752.2003

Figure Lengend Snippet: Growth of L. fermentum KC5b in MRS-C broth containing 1% (wt/vol) of glucose (▪), GOS (□), FOS (⧫), or lactulose (▵). Each point represents the mean of three independent trials; error bars represent standard deviations.

Article Snippet: These results are shown in Table . table ft1 table-wrap mode="anchored" t5 TABLE 3. caption a7 Bile acid Amount (μmol ± SD) of CA formed by: L. fermentum KC5b S. bovis ATCC 43143 E. faecalis UK873 TCA 3.0 ± 1.2 30.9 ± 1.0 9.5 ± 6.6 GCA 3.1 ± 2.0 35.5 ± 1.3 23.9 ± 5.2 Open in a separate window a Values calculated on the basis of growth in MRS broth supplemented with 1 mM GCA and 1 mM TCA.

Techniques:

Journal:

Article Title: An In Vitro Study of the Probiotic Potential of a Bile-Salt-Hydrolyzing Lactobacillus fermentum Strain, and Determination of Its Cholesterol-Lowering Properties

doi: 10.1128/AEM.69.8.4743-4752.2003

Figure Lengend Snippet: Influence of oxgall concentration on growth rate (▪) and cholesterol reduction (▵) of L. fermentum KC5b. Levels of cholesterol removed from the culture broth are expressed as mean percentages of the initial concentration during the 24-h incubation. Error bars represent standard deviations (n = 2).

Article Snippet: These results are shown in Table . table ft1 table-wrap mode="anchored" t5 TABLE 3. caption a7 Bile acid Amount (μmol ± SD) of CA formed by: L. fermentum KC5b S. bovis ATCC 43143 E. faecalis UK873 TCA 3.0 ± 1.2 30.9 ± 1.0 9.5 ± 6.6 GCA 3.1 ± 2.0 35.5 ± 1.3 23.9 ± 5.2 Open in a separate window a Values calculated on the basis of growth in MRS broth supplemented with 1 mM GCA and 1 mM TCA.

Techniques: Concentration Assay, Incubation

Journal:

Article Title: An In Vitro Study of the Probiotic Potential of a Bile-Salt-Hydrolyzing Lactobacillus fermentum Strain, and Determination of Its Cholesterol-Lowering Properties

doi: 10.1128/AEM.69.8.4743-4752.2003

Figure Lengend Snippet: Cholesterol reduction by L. fermentum KC5b in the presence of different bile salts a

Article Snippet: These results are shown in Table . table ft1 table-wrap mode="anchored" t5 TABLE 3. caption a7 Bile acid Amount (μmol ± SD) of CA formed by: L. fermentum KC5b S. bovis ATCC 43143 E. faecalis UK873 TCA 3.0 ± 1.2 30.9 ± 1.0 9.5 ± 6.6 GCA 3.1 ± 2.0 35.5 ± 1.3 23.9 ± 5.2 Open in a separate window a Values calculated on the basis of growth in MRS broth supplemented with 1 mM GCA and 1 mM TCA.

Techniques:

Journal:

Article Title: Cholesterol Assimilation by Lactic Acid Bacteria and Bifidobacteria Isolated from the Human Gut

doi: 10.1128/AEM.68.9.4689-4693.2002

Figure Lengend Snippet: Origin and source of bacterial strains a

Article Snippet: However, this effect was not statistically significant ( P > 0.05). table ft1 table-wrap mode="anchored" t5 TABLE 2. caption a7 Strain Cholesterol uptake (mg of cholesterol g [dry wt]) 0.2% oxgall 0.4% oxgall Run 1 Run 2 Run 3 Run 1 Run 2 Run 3 L. brevis NR1C1684 8.6 (0.5) 19 (1) 3.9 (0.6) 1.6 (0.1) 8.0 (0.8) 13.2 (0.7) L. pentosus (A) 9.4 (0.5) 0.88 (0.05) 0.10 (0.03) 6.9 (0.4) 16.6 (0.9) 15.3 (0.8) L. plantarum NDV 0.92 (0.07) 6.9 (0.4) 0.02 (0.04) 5.5 (0.3) 11.1 (0.6) 16.4 (0.9) L. reuteri JCM 1112 0.09 (0.08) 10.6 (0.6) 4.8 (0.3) 10.4 (0.5) 12.5 (0.7) 11.3 (0.6) L. casei Shirota 5.5 (0.3) 6.0 (0.3) 1.14 (0.07) 8.1 (0.4) 8.7 (0.5) 9.5 (0.5) L. casei 2.9 (0.2) 3.7 (0.2) 0.40 (0.05) 14.1 (0.7) 3.0 (0.2) 8.5 (0.4) L. acidophilus johnsonii 3.0 (0.2) 0.22 (0.02) 1.47 (0.08) 3.3 (0.2) 0.94 (0.3) 11.7 (0.6) L. delbrueckii JCM 1002 4.9 (0.3) 3.8 (0.2) 6.7 (0.4) 13.7 (0.7) 2.5 (0.2) 13.9 (0.7) L. fermentum F53 0.39 (0.06) 6.2 (0.3) 0.77 (0.05) 12.9 (0.7) 6.9 (0.4) 8.8 (0.5) L. fermentum KC5B 8.3 (0.4) 2.4 (0.1) 2.4 (0.1) 14.8 (0.8) 5.3 (0.3) 4.1 (0.2) S. thermophilus DSM 20617 3.7 (0.1) ND ND 6.1 (0.2) ND ND S. bovis ATCC 43143 2.47 (0.09) 6.4 (0.2) 1.55 (0.07) 6.6 (0.2) 6.7 (0.2) 20.0 (0.7) L. pentosus (B) 2.4 (0.1) 10.5 (0.6) 2.6 (0.2) 6.5 (0.3) 22 (1) 26 (1) L. reuteri DSM 20016T 2.1 (0.1) 0.84 (0.07) 0.64 (0.05) 5.2 (0.3) 23 (1) 10.7 (0.6) E. durans DSM 20633 6.2 (0.4) 0.66 (0.07) 0.45 (0.05) 4.8 (0.3) 26 (1) 19 (1) E. gallinarum 7.1 (0.4) 0.30 (0.09) 0.28 (0.07) 8.3 (0.5) 27 (1) 19 (1) E. faecalis 6.5 (0.4) 5.3 (0.3) 3.6 (0.2) 1.50 (0.09) 30 (1) 26 (2) B. infantis ATCC 15697 1.31 (0.05) 3.9 (0.1) 4.7 (0.2) 9.6 (0.3) 7.3 (0.3) 4.3 (0.2) B. infantis D86184 4.3 (0.4) 2.0 (0.2) 6.1 (0.3) ND ND ND Open in a separate window a Results are shown as means (standard deviations) ( n = 3).

Techniques:

Journal: Clinical and Experimental Gastroenterology

Article Title: Quantification of Intestinal Lactobacillus Species in Children with Functional Constipation by Quantitative Real-Time PCR

doi: 10.2147/CEG.S250755

Figure Lengend Snippet: Specificity of the L. Casei Primer Pair Used in This Study

Article Snippet: Seven Lactobacillus reference strains ( L. casei ATCC 39392, L. paracasei ATCC 25598, L. rhamnosus ATCC 7469, L. plantarum ATCC 8014, L. reuteri ATCC 23272, L. fermentum ATCC 9338, L. acidophilus ATCC 4356) used to validate the assays in the study were ordered from the Iranian Research Organization for Science and Technology (IROST, Iran).

Techniques: Real-time Polymerase Chain Reaction

Journal: Clinical and Experimental Gastroenterology

Article Title: Quantification of Intestinal Lactobacillus Species in Children with Functional Constipation by Quantitative Real-Time PCR

doi: 10.2147/CEG.S250755

Figure Lengend Snippet: Prevalence of Lactobacillus Species, Detected by Quantitative PCR

Article Snippet: Seven Lactobacillus reference strains ( L. casei ATCC 39392, L. paracasei ATCC 25598, L. rhamnosus ATCC 7469, L. plantarum ATCC 8014, L. reuteri ATCC 23272, L. fermentum ATCC 9338, L. acidophilus ATCC 4356) used to validate the assays in the study were ordered from the Iranian Research Organization for Science and Technology (IROST, Iran).

Techniques:

Journal: Clinical and Experimental Gastroenterology

Article Title: Quantification of Intestinal Lactobacillus Species in Children with Functional Constipation by Quantitative Real-Time PCR

doi: 10.2147/CEG.S250755

Figure Lengend Snippet: Quantity of seven Lactobacillus species in the feces of constipated children and healthy controls. Statistical significance of observed differences in the amount of Lactobacillus species between both constipated and healthy groups was measured by the Mann–Whitney U -test. Bars represent standard errors. P < 0.05 was marked with one asterisk (∗), and P < 0.2 with two asterisks (∗∗). One-way ANOVA testing shows the significant differences in quantity (log 10 CFU/gram) between Lactobacillus species.

Article Snippet: Seven Lactobacillus reference strains ( L. casei ATCC 39392, L. paracasei ATCC 25598, L. rhamnosus ATCC 7469, L. plantarum ATCC 8014, L. reuteri ATCC 23272, L. fermentum ATCC 9338, L. acidophilus ATCC 4356) used to validate the assays in the study were ordered from the Iranian Research Organization for Science and Technology (IROST, Iran).

Techniques: MANN-WHITNEY